High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied ...

Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most ...