Now showing items 20-37 of 37

    • In vivo assessment of closantel ovicidal activity in Fasciola hepatica eggs. 

      Solana, María Victoria; Mera y Sierra, Roberto; Scarcella, Silvana; Neira, Gisela; Solana, Hugo Daniel (Editorial Experimental Parasitology, 2016)
      Anthelmintic resistance in livestock parasites is currently a worldwide problem. Fasciola hepatica is a cosmopolitan parasite which causes considerable loss in sheep and cattle production systems all over the world. Chemotherapy is currently the main tool available for its control. The intensive use of triclabendazole, the drug of choice for more than 20 years, has resulted in the development of resistant strains. The therapeutic options are adulticides such as closantel (salicylanilide anthelmintic that binds extensively to plasma albumin) to treat chronic fascioliasis in sheep, and cattle. In ...
    • Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner 

      Gimenez, María Cecilia; Rodríguez Aguirre, José Francisco; Colombo, María Isabel; Delgui, Laura Ruth (Comité editorial Cellular Microbiology, 2015-01-07)
      Infectious bursal disease virus (IBDV) internalization is sparsely known in terms of molecular components of the pathway involved. To describe the cell biological features of IBDV endocytosis, we employed perturbants of endocytic pathways such as pharmacological inhibitors and overexpression of dominant-negative mutants. Internalization analysis was performed quantifying infected cells by immunofluorescence and Western blot detection of the viral protein VP3 at 12 h post-infection reinforced by the analysis of the capsid protein VP2 localization after virus uptake at 1 h post-infection. ...
    • Inhibición de butirilcolinesterasa en dos perros intoxicados y confirmación analítica de carbofuran como agente causal 

      Ferré, Daniela Marisol; Saldeña, Eliana Leticia; Albarracín, Lázaro; Neuilly, Verónica; Gorla, Nora Bibiana María (Comité editorial Revista Veterinaria, Facultad de Ciencias Veterinarias de la Universidad Nacional del Nordeste (UNNE), 2015)
      La mortandad de animales domésticos y silvestres debido a intoxicaciones por plaguicidas es internacionalmente elevada, en la mayoría de los casos por exposición a insecticidas. Los más comúnmente citados son los anticolinesterásicos organofosforados y carbamatos. Los perros y gatos mascotas son buenos recursos para alertar sobre el uso de venenos en el medio natural y los posibles riesgos para el hombre con el que comparten el ambiente. En dos perros con signos de intoxicación, el objetivo del presente trabajo fue efectuar estudios complementarios de laboratorio para identificar con certeza ...
    • Invasive pulmonary aspergillosis in a captive bird Cyanocompsa brissonii (Cardinalidae) in Mendoza, Argentina 

      Palma Leotta, María; Pelegrina, Marisa; Cáceres, Antonella (Comité editorial Revista Veterinaria, Facultad de Ciencias Veterinarias de la Universidad Nacional del Nordeste (UNNE), 2015)
      Se reporta un caso de aspergillosis pulmonar en un ejemplar cautivo de Cyanocompsa brissonii (“reinamora grande”). Las lesiones observadas sugieren la forma infiltrativa o superficial difusa de la enfermedad, típica de las serosas y pulmones. El contagio y posterior muerte del animal podrían haber sido el resultado de una serie de factores que afectaron su inmunidad y predispusieron a la micosis. El organismo encontrado fue identificado como Aspergillus fumigatus, en base a la observación microscópica de conidióforos característicos. Desde el punto de vista epidemiológico, C. brissonii debería ...
    • Liver fluke (Fasciola hepatica) naturally infecting introduced European brown hare (Lepus europaeus) in northern Patagonia: phenotype, prevalence and potential risk 

      Cuervo, Pablo Fernando; Di Cataldo, Sophia; Fantozzi, María Cecilia; Deis, Erika; Diaz Isenrath, Gabriela; Viberti, Gabriela; Artigas, Patricio; Peixoto, Raquel; Valero, María Adela; Mera y Sierra, Roberto; Mas-Coma, Santiago (Comité editorial Acta Parasitologica, 2015-06-23)
      Fascioliasis has recently been included in the WHO list of Neglected Zoonotic Diseases. Besides being a major veterinary health problem, fascioliasis has large underdeveloping effects on the human communities affected. Though scarcely considered in fascioliasis epidemiology, it is well recognized that both native and introduced wildlife species may play a significant role as reservoirs of the disease. The objectives are to study the morphological characteristics of Fasciola hepatica adults and eggs in a population of Lepus europaeus, to assess liver fluke prevalence, and to analyze the potential ...
    • Ocurrencia de enfermedades transmitidas por vectores en caninos domésticos de San José, Guaymallén 

      Cuervo, Pablo; Di Cataldo, Sophía; Rodríguez, María Belén; Fantozzi, Cecilia; Pedrosa, Analía; Lencinas, Patricia; Mera y Sierra, Roberto (Dirección de Investigación, Ciencia y Técnica. Ministerio de Salud. Mendoza, 2015)
      Boletines de la Dirección de Investigación, Ciencia y Técnica del Ministerio de Salud de Mendoza. Los artículos publicados pertenecen a resultados de proyectos UMaza con subsidio de tal Dirección a través de la convocatoria "Investigadores Mendocinos". Instituciones de los autores: Centro de Investigación en Parasitología Regional (CIPAR), Universidad Juan Agustín Maza; Departamento de Enfermedades Zoonóticas y Vectoriales, Ministerio de Salud, Mendoza; Laboratorio de Biopatología, Universidad Juan Agustín Maza; Laboratorio de Genética, Ambiente y Reproducción (GenAR), Universidad Juan Agustín ...
    • Plasma Cholinesterase Activity in Wild Birds from Undisturbed Woodlands in the Central Monte Desert 

      Quero, Arnoldo Ängel Martín; Zarco, Agustín; Landa, Florencia Belén; Gorla, Nora Bibiana María (2019-04-30)
      Plasma cholinesterase activity is a biomarker sensitive to the effect of organophosphate and carbamate pesticides, and its enzymatic levels have been previously unknown for most of the wild birds analyzed in the present study. Our objectives were to establish plasma acetylcholinesterase levels in songbirds of 2 undisturbed sites in the central Monte Desert (Argentina). We also examined the influence on cholinesterase activity of age, sex, body condition, feeding and migratory habits, and species. One hundred and sixty‐five wild birds belonging to 26 species were studied. The values obtained for ...
    • Potential risks of dietary exposure to chlorpyrifos and cypermethrin from their use in fruit/vegetable crops and beef cattle productions. 

      Ferré, ´Daniela Marisol; Quero, Arnoldo Ángel Martín; Hernández, Antonio Francisco; Hynes, Valentina; Tornello, Marcelo Javier; Lúders, Carlos; Gorla, Nora Bibiana María (Comité editorial Environ Monitoring and Assessment, 2018-04-18)
      The active ingredients (a.i.) used as pesticides vary across regions. Diet represents the main source of chronic exposure to these chemicals. The aim of this study was to look at the pesticides applied in fruit, vegetable, and beef cattle productions in Mendoza (Argentina), to identify those that were simultaneously used by the three production systems. Local individuals (n = 160), involved in these productions, were interviewed. Glyphosate was the a.i. most often used by fruit-vegetable producers, and ivermectin by beef cattle producers. Chlorpyrifos (CPF) and cypermethrin (CYP) were the only ...
    • Pseudobutyrivibrio xylanivorans adhesion to epithelial cells 

      Grilli, Diego Javier; Mansilla, María Eugenia; Gimenez, María Cecilia; Sohaefer, Noelia; Ruiz, María Soledad; Terebiznik, Mauricio; Sosa, Miguel; Arenas, Graciela Nora (Comité editorial Anaerobe Journal, 2019-01-05)
      The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the major requirements for probiotics. In this study, we assessed the adhesion of P. xylanivorans 2 to SW480 cells and characterized this process utilizing multiple microscopy approaches. Our results indicate that a multiplicity of infection of 200 CFU/cell allows the highest bacteria to cell binding ratio, with a lower percentage of ...
    • Reference values for acetyl and butyrylcholinesterases in cattle under actual management conditions, hepatic and renal function by application of chlorpyrifos 

      Ferré, Daniela Marisol; Lentini, Valeria Roxana; Romano, Raquel; Ludueña, Héctor Ricardo; Jotallán, Paola Jimena; Gorla, Nora Bibiana María (Comité editorial Journal of Environmental Science and Health, 2018-01-02)
      Chlorpyrifos is an anticholinesterase organophosphate insecticide widely used in Argentina in the production of food derived from animal, fruit and horticultural origin and is reported as a residue within these products. Local reference values for acetyl and butyrylcholinesterase were determined in Aberdeen Angus bovine and cross bred cattle (n D 25), a requirement to be able to evaluate toxicity of commercial organophosphate and carbamate formulations. The activity of cholinesterase enzymes presented an overall mean of 2,183.00 § 485.6 IU L¡1 for erythrocyte acetylcholinesterase and 203.1 § ...
    • Report of Amblyomma parvitarsum infesting goats in Midwestern Argentina 

      Fantozzi, María Cecilia; Tarragona, Evelina Luisa; Dayenoff, Patricio; Cuervo, Pablo Fernando; Mera y Sierra, Roberto; Nava, Santiago (Comité editorial Veterinary Parasitology, 2018-09-24)
      Adult ticks were collected from goats on September 2012 in the locality of Trintrica (35° 17′ 19″ S - 68° 44′ 6″ W, 1430 m.a.s.l.), Mendoza, Argentina. The specimens were identified as seven females and three males of Amblyomma parvitarsum Neumann, 1901. This record represents the new occurrence of A. parvitarsum in the province of Mendoza, as well as the first report of the parasitism in goats along the geographical range of A. parvitarsum.
    • Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. 

      Henderson, Gemma; Cox, Faith; Ganesh, Siva; Jonker, Arjan; Young, Wayne; Global Rumen Census Collaborators; Janssen, Peter (Comité editorial Scientific Reports, 2015-10-09)
      Ruminant livestock are important sources of human food and global greenhouse gas emissions. Feed degradation and methane formation by ruminants rely on metabolic interactions between rumen microbes and affect ruminant productivity. Rumen and camelid foregut microbial community composition was determined in 742 samples from 32 animal species and 35 countries, to estimate if this was influenced by diet, host species, or geography. Similar bacteria and archaea dominated in nearly all samples, while protozoal communities were more variable. The dominant bacteria are poorly characterised, but the ...
    • Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. 

      Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Kateřina; Kopečný, Jan; Paez Lama, Sebastian; Cerón Cucchi, María Esperanza; Sosa, Miguel Angel; Arenas, Graciela Nora (Editorial Livestock Science, 2016-08-02)
      Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most ...
    • Seasonal changes in feed intake, diet digestibility and diet composition by lactating and non-lactating goats browsing in a semi-arid rangeland of Argentina 

      Egea, Angela Vanina; Bakker, María Leontina; Allegretti, Liliana Inés; Paez, Sebastián Antonio; Grilli, Diego Javier; Guevara, Juan Carlos; Viillalba, Juan José (Comité editor Grass and Forage Science, 2018-10-01)
      We determined the seasonal nutrient and secondary compound intake, in vivo diet digestibility (DD) and diet species composition of goats in different physiological states in a desert rangeland of Argentina. During the wet and the dry seasons, 24 mature female Creole goats (six non-lactating, non-pregnant and six lactating in each season) were used in a 2 × 2 factorial design. Diet botanical composition, DD and dry-matter intake (DMI) were estimated by the combined use of visual observation, microhistological analyses of faecal samples and n-alkane technique. Browse species dominated the goats’ ...
    • A Single Nucleotide Polymorphism Translates into a Radical Amino Acid Substitution at the Ligand-Binding Site in Fasciola hepatica Carboxylesterase B 

      Miranda-Miranda, Estefan; Scarcella, Silvana; Reynaud, Enrique; Narváez-Padilla, Verónica; Neira, Gisela; Mera y Sierra, Roberto; Aguilar-Díaz, Hugo; Cossio-Bayugar, Raquel (Comite editorial GENES, 2022-10-19)
      Fasciola hepatica anthelmintic resistance may be associated with the catalytic activity of xenobiotic metabolizing enzymes. The gene expression of one of these enzymes, identified as car boxylesterase B (CestB), was previously described as inducible in adult parasites under anthelmintic treatment and exhibited a single nucleotide polymorphism at position 643 that translates into a radical amino acid substitution at position 215 from Glutamic acid to Lysine. Alphafold 3D models of both allelic sequences exhibited a significant affinity pocket rearrangement and different ligand-docking modeling ...
    • Situación de la fasciolasis en Mendoza: nuevos desafíos de una enfermedad desatendida 

      Mera y Sierra, Roberto; Cuervo, Pablo; Sidoti, Laura; Deis, Erika; Fantozzi, Cecilia; Sohaefer, Noelia; Neira, Gisela; Di Cataldo, Sophía; Spongia, Silvina; Gerbeno, Leticia (Dirección de Investigación, Ciencia y Técnica. Ministerio de Salud. Mendoza, 2013-03)
      Boletines de la Dirección de Investigación, Ciencia y Técnica del Ministerio de Salud de Mendoza. Los artículos publicados pertenecen a resultados de proyectos UMaza con subsidio de tal Dirección a través de la convocatoria "Investigadores Mendocinos".
    • Unusual presence of Ornidia robusta (Diptera: Syrphidae) causing pig myiasis in Argentina. 

      López Millán, Cyntia; Olea, María Sofía; Dantur Juri, María Julia (Comité editorial Parasitology Research, 2015-10-09)
      Myiasis is caused by dipterous larvae from the Calliphoridae, Sarcophagidae, Muscidae, Cuterebridae, and Syrphidae families. In this work, Cochliomyia hominivorax, Chrysomya megacephala, and Ornidia robusta were identified causing vulva, ear, and leg myiasis in pigs in Tucuman province, northwestern Argentina. The report of the presence of C. hominivorax and C. megacephala is very important due to their role as myiasis-causing and disease vectors. The occurrence of Ornidia robusta is remarkable, since it constitutes the first record of myiasis in general and of myiasis in pigs in particular. ...
    • Vector Borne Diseases in Mid Western Argentina, First Report of Setaria Equina [Nematoda: Onchocercidae] in A Horse [Equus caballus] 

      Mera y Sierra, Roberto; Iranzo, José; Neira, Gisela (Comité editorial Austin Journal of Vector Borne Diseases, 2017-12-05)
      ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Vector borne diseases are spreading worldwide. Companion animals can be useful as sentinels for disease surveillance. Setaria spp. are filarial nematodes transmitted by Aedes spp. and Culex spp. Setaria equina has been reported in Asia, Africa and North America. This zoonotic nematode is frequently asymptomatic in horses, except during its erratic migrations. Filarial worms have a tropism for ocular and nervous tissues, having important implications in veterinary and public health. In mid west Argentina, Dirofilaria immitis has already been described ...