Now showing items 1-4 of 4

    • Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet. 

      Grilli, Diego Javier; Fliegerová, Kateřina; Kopečný, Jan; Paez Lama, Sebastián; Egea, Vanina; Sohaefer, Noelia; Pereyra, Celia; Ruiz, María Soledad; Sosa, Miguel Angel; Arenas, Graciela Nora; Mrázek, Jakub (Comité editor Journal Anaerobe, 2016-07-12)
      ARTÍCULO PUBLICADO EN REVISTA EXTERNA. High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal ...
    • Degradación y utilización de la hemicelulosa contenida en especies forrajeras por Pseudobutyrivibrio ruminis y Pseudobutyrivibrio xylanivorans 

      Grilli, Diego; Egea, Vanina; Paez Lama, Sebastián; Carcaño, Diego; Allegretti, Liliana; Sosa Escudero, Miguel; Arenas, Graciela Nora (Comité editorial Revista de la Facultad de Ciencias Agrarias, 2015-12)
      ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Este estudio fue desarrollado para determinar la degradación y utilización de la hemicelulosa contenida en diferentes forrajes por dos cepas bacterianas (P. xylanivorans 2 y P. ruminis 153), aisladas del rumen de caprinos Criollos. El cultivo de las cepas puras fue desarrollado hasta evidenciar la fermentación completa del heno de alfalfa y de diversas forrajeras nativas. La degradación fue definida como la solubilización de las pentosas insolubles en etanol al 80%, mientras que la utilización se definió como la pérdida de las pentosas totales. Los componentes ...
    • Pseudobutyrivibrio xylanivorans adhesion to epithelial cells 

      Grilli, Diego Javier; Mansilla, María Eugenia; Gimenez, María Cecilia; Sohaefer, Noelia; Ruiz, María Soledad; Terebiznik, Mauricio; Sosa, Miguel; Arenas, Graciela Nora (Comité editorial Anaerobe Journal, 2019-01-05)
      ARTÍCULO PUBLICADO EN REVISTA EXTERNA. The ruminal bacteria Pseudobutyrivibrio xylanivorans strain 2 (P. xylanivorans 2), that mediates the digestion of plant fiber, is considered an attractive candidate for probiotics. Adherence to the epithelium of the digestive tract of the host is one of the major requirements for probiotics. In this study, we assessed the adhesion of P. xylanivorans 2 to SW480 cells and characterized this process utilizing multiple microscopy approaches. Our results indicate that a multiplicity of infection of 200 CFU/cell allows the highest bacteria to cell binding ...
    • Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. 

      Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Kateřina; Kopečný, Jan; Paez Lama, Sebastian; Cerón Cucchi, María Esperanza; Sosa, Miguel Angel; Arenas, Graciela Nora (Editorial Livestock Science, 2016-08-02)
      ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not ...