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dc.contributor.authorGrilli, Diego Javier
dc.contributor.authorMrázek, Jakub
dc.contributor.authorFliegerová, Kateřina
dc.contributor.authorKopečný, Jan
dc.contributor.authorPaez Lama, Sebastian
dc.contributor.authorCerón Cucchi, María Esperanza
dc.contributor.authorSosa, Miguel Angel
dc.contributor.authorArenas, Graciela Nora
dc.date.accessioned2020-10-06T13:39:04Z
dc.date.available2020-10-06T13:39:04Z
dc.date.issued2016-08-02
dc.identifier.citationGrili, D. J., Mrázek, J., Fliegerová, K., Kopečný, J., Paez Lama, S., Cerón Cucchi, M. E., Sosa, M. A., y Arenas, G. N. (2016) Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. Livestock Science, 191, 191-195. https://doi.org/10.1016/j.livsci.2016.08.001en_US
dc.identifier.issn1871-1413
dc.identifier.urihttp://repositorio.umaza.edu.ar//handle/00261/1747
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S187114131630172X#!
dc.description.abstractUsing culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.en_US
dc.language.isoengen_US
dc.publisherEditorial Livestock Scienceen_US
dc.source191;
dc.subjectBacteriasen_US
dc.subjectCreole Goatsen_US
dc.subjectQPCRen_US
dc.subjectPCR-DGGEen_US
dc.subjectRumensen_US
dc.titleRuminal bacterial community changes during adaptation of goats to fresh alfalfa forage.en_US
dc.typeInstitucional - Material de Difusiónen_US
umaza.description.filiationFil: Grilli, Diego Javier. Facultad de Ciencias Veterinarias y Ambientales, Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519 Mendoza, Argentina. Instituto de Histología y Embriología de Mendoza, Universidad Nacional de Cuyo, Casilla de Correo 56, CP 5500 Mendoza, Argentina.en_US
umaza.description.filiationFil: Mrázek, Jakub. Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Videnska 1083, CP 142 20 Prague, Czech Republic.en_US
umaza.description.filiationFil: Fliegerováb, Kateřina. Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Videnska 1083, CP 142 20 Prague, Czech Republic.en_US
umaza.description.filiationFil: Kopečný, Jan. Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i., Videnska 1083, CP 142 20 Prague, Czech Republic.en_US
umaza.description.filiationFil: Paez Lama, Sebastian. Instituto Argentino de Investigaciones de las Zonas Áridas, Centro Científico y Tecnológico, M5502BPB Mendoza, Argentina.en_US
umaza.description.filiationFil: Cerón Cucchi, María Esperanza. Instituto de Patobiología. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto Nacional de Tecnología Agropecuaria. De Los Reseros y Dr. Nicolás Repetto S/N, Hurlingham, CP 1686 Buenos Aires, Argentina.en_US
umaza.description.filiationFil: Sosa, Miguel Ángel. Instituto de Histología y Embriología de Mendoza, Universidad Nacional de Cuyo, Casilla de Correo 56, CP 5500 Mendoza, Argentina.en_US
umaza.description.filiationFil: Arenas, Graciela Nora. Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Casilla de Correo 56, CP 5500 Mendoza, Argentina.en_US
umaza.statusSNRDPublicadaen_US


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