Infectious bursal disease virus uptake involves macropinocytosis and trafficking to early endosomes in a Rab5-dependent manner
Date
2015-01-07Author
Gimenez, María Cecilia
Rodríguez Aguirre, José Francisco
Colombo, María Isabel
Delgui, Laura Ruth
Metadata
Show full item recordAbstract
ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Infectious bursal disease virus (IBDV) internalization
is sparsely known in terms of molecular components
of the pathway involved. To describe
the cell biological features of IBDV endocytosis,
we employed perturbants of endocytic pathways
such as pharmacological inhibitors and overexpression
of dominant-negative mutants. Internalization
analysis was performed quantifying
infected cells by immunofluorescence and
Western blot detection of the viral protein VP3 at
12 h post-infection reinforced by the analysis of
the capsid protein VP2 localization after virus
uptake at 1 h post-infection. We compared IBDV
infection to the internalization of well-established
ligands with defined endocytic pathways: transferrin,
cholera-toxin subunit B and dextran. To
describe virus endocytosis at the morphological
level, we performed ultrastructural studies of
viral internalization kinetics in control and actin
dynamics-blocked cells. Our results indicate that
IBDV endocytic internalization was clathrin- and
dynamin-independent, and that IBDV uses macropinocytosis
as the primary entry mechanism. After uptake, virus traffics to early endosomes
and requires exposure to the low endocytic pH as
well as a functional endocytic pathway to complete
its replication cycle. Moreover, our results
indicate that the GTPase Rab5 is crucial for IBDV
entry supporting the participation of the early
endosomal pathway in IBDV internalization and
infection of susceptible cells. Sitio de la revista: https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12415 Fil: Gimenez, María Cecilia. Facultad de Farmacia y Bioquímica, Universidad Juan Agustín Maza, Mendoza, Argentina. Instituto de Histología y Embriología (IHEM), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo – CONICET, Mendoza, Argentina. Fil: Rodríguez Aguirre, José Francisco. 3Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología – CSIC, Madrid, Spain. Fil: Colombo, María Isabel. Facultad de Farmacia y Bioquímica, Universidad Juan Agustín Maza, Mendoza, Argentina. Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología – CSIC, Madrid, Spain. Fil: Delgui, Laura Ruth. Facultad de Farmacia y Bioquímica, Universidad Juan Agustín Maza, Mendoza, Argentina. Instituto de Histología y Embriología (IHEM), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo – CONICET, Mendoza, Argentina. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Mendoza, Argentina.
URI
http://repositorio.umaza.edu.ar//handle/00261/1816https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12415