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dc.contributor.authorFerré, Daniela Marisol
dc.contributor.authorLudueña, Héctor Ricardo
dc.contributor.authorRomano, Raquel
dc.contributor.authorGorla, Nora Bibiana María
dc.date.accessioned2020-11-07T10:08:18Z
dc.date.available2020-11-07T10:08:18Z
dc.date.issued2019-11-09
dc.identifier.citationFerré, D. M. Ludueña, H. R., Romano, R., y Gorla, N. B. M. (2019) Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes. Chemosphere, 243. https://doi.org/10.1016/j.chemosphere.2019.125341en_US
dc.identifier.issn0045-6535
dc.identifier.urihttp://repositorio.umaza.edu.ar//handle/00261/1823
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0045653519325810#!
dc.description.abstractCypermethrin (CYP) and chlorpyrifos (CPF) are insecticides/parasiticides used in the production of fruits, vegetables and beef cattle. These substances or their metabolites are frequently reported as residues in food, whose consumption in a diet implies a genotoxic risk. The potential for chronic toxicity of CYP and CPF is unclear, and only a few genotoxicological evaluations based on their mixture have been performed. The aim of this study was to evaluate the genotoxic potential of CYP, CPF and CYP þ CPF in five concentrations, from 5.9 to 175 mg/mL, on bovine lymphocytes. By means of the cytokinesis-block micronucleus cytome assay, a decrease in the cell proliferation index was observed (r ¼ 0.89 p ¼ 0.04); and also an increase in the frequencies of binucleated cells (BN) with micronuclei (BNMn) (r ¼ 0.93, p ¼ 0.02) and BN with nuclear buds (BNBud) (r ¼ 0.778 p ¼ 0.04), depending on the concentrations of CPF. An increase in BNMn frequencies was observed as a function of CYP concentrations (r ¼ 0.89, p ¼ 0.04) and also of the CYP þ CPF mix (r ¼ 0.99, p ¼ 0.008). CYP caused greater genotoxic damage (BNMn) than CPF and the mixture on bovine lymphocytes. Cells with simultaneous presentation of micronuclei and nuclear buds were detected, as well as cells with irregular nuclei, something never previously reported, whose origin and significance should be investigated. The genotoxic effect of chlorpyrifos, cypermethrin and their mixture on bovine lymphocytes was observed. We recognized the value of the use of primary bovine cultures, animal species adjacent to man in the food chain, for genotoxicity studies.en_US
dc.language.isoengen_US
dc.publisherComité editorial Chemosphere Journalen_US
dc.source243;
dc.subjectCypermethrinen_US
dc.subjectChlorpyrifosen_US
dc.subjectMicronucleusen_US
dc.subjectCell proliferation indexen_US
dc.subjectParasiticidesen_US
dc.subjectCBMN Assayen_US
dc.titleEvaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytesen_US
dc.typeInstitucional - Material de Difusiónen_US
umaza.description.filiationFil: Ferré, Daniela Marisol. Facultad de Ciencias Veterinarias y Ambientales, Universidad Juan Agustín Maza, Mendoza, Argentina.onsejo Nacional de Investigaciones Científicas y Técnicas -CONICET, Mendoza, Argentina.en_US
umaza.description.filiationFil: Ludueña, Héctor Ricardo. Laboratorio de Genética, Ambiente y Reproducción, Universidad Juan Agustín Maza, Mendoza, Argentinaen_US
umaza.description.filiationFil: Romano, Raquel. Laboratorio de Genética, Ambiente y Reproducción, Universidad Juan Agustín Maza, Mendoza, Argentinaen_US
umaza.description.filiationFil: Gorla, Nora Bibiana María. Facultad de Ciencias Veterinarias y Ambientales, Universidad Juan Agustín Maza, Mendoza, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas -CONICET, Mendoza, Argentina.en_US
umaza.statusSNRDPublicadaen_US


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