Evaluation of the genotoxic potential of cypermethrin, chlorpyrifos and their subsequent mixture, on cultured bovine lymphocytes
Ferré, Daniela Marisol
Ludueña, Héctor Ricardo
Gorla, Nora Bibiana María
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ARTÍCULO PUBLICADO EN REVISTA EXTERNA. Cypermethrin (CYP) and chlorpyrifos (CPF) are insecticides/parasiticides used in the production of fruits, vegetables and beef cattle. These substances or their metabolites are frequently reported as residues in food, whose consumption in a diet implies a genotoxic risk. The potential for chronic toxicity of CYP and CPF is unclear, and only a few genotoxicological evaluations based on their mixture have been performed. The aim of this study was to evaluate the genotoxic potential of CYP, CPF and CYP þ CPF in five concentrations, from 5.9 to 175 mg/mL, on bovine lymphocytes. By means of the cytokinesis-block micronucleus cytome assay, a decrease in the cell proliferation index was observed (r ¼ 0.89 p ¼ 0.04); and also an increase in the frequencies of binucleated cells (BN) with micronuclei (BNMn) (r ¼ 0.93, p ¼ 0.02) and BN with nuclear buds (BNBud) (r ¼ 0.778 p ¼ 0.04), depending on the concentrations of CPF. An increase in BNMn frequencies was observed as a function of CYP concentrations (r ¼ 0.89, p ¼ 0.04) and also of the CYP þ CPF mix (r ¼ 0.99, p ¼ 0.008). CYP caused greater genotoxic damage (BNMn) than CPF and the mixture on bovine lymphocytes. Cells with simultaneous presentation of micronuclei and nuclear buds were detected, as well as cells with irregular nuclei, something never previously reported, whose origin and significance should be investigated. The genotoxic effect of chlorpyrifos, cypermethrin and their mixture on bovine lymphocytes was observed. We recognized the value of the use of primary bovine cultures, animal species adjacent to man in the food chain, for genotoxicity studies. Sitio de la revista: https://www.sciencedirect.com/science/article/abs/pii/S0045653519325810#!Fil: Ferré, Daniela Marisol. Facultad de Ciencias Veterinarias y Ambientales, Universidad Juan Agustín Maza, Mendoza, Argentina.onsejo Nacional de Investigaciones Científicas y Técnicas -CONICET, Mendoza, Argentina.Fil: Ludueña, Héctor Ricardo. Laboratorio de Genética, Ambiente y Reproducción, Universidad Juan Agustín Maza, Mendoza, ArgentinaFil: Romano, Raquel. Laboratorio de Genética, Ambiente y Reproducción, Universidad Juan Agustín Maza, Mendoza, ArgentinaFil: Gorla, Nora Bibiana María. Facultad de Ciencias Veterinarias y Ambientales, Universidad Juan Agustín Maza, Mendoza, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas -CONICET, Mendoza, Argentina.