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dc.contributor.authorPelegrina, Laura Tatiana
dc.contributor.authorSanhuesa, María de los Ángeles
dc.contributor.authorCáceres, Antonella Rosario Ramona
dc.contributor.authorCuello Carrión, Dario
dc.contributor.authorRodríguez, Cristina Elisa
dc.contributor.authorLaconi, Myriam Raquel
dc.date.accessioned2020-08-29T16:45:46Z
dc.date.available2020-08-29T16:45:46Z
dc.date.issued2020-02
dc.identifier.citationPelegrina, L., T., Sanhueza, M. de los A., Cáceres, A. R. R., Cuello Carrion, D., Rodriguez, C. E. y Laconi, M. R. (2020). Effect of progesterone and first evidence about allopregnanolone action on the progression of epithelial human ovarian cancer cell lines. Journal of Steroid Biochemistry and Molecular Biology, 196. Disponible en: https://www.sciencedirect.com/science/article/pii/S0960076019301013?via%3Dihuben_US
dc.identifier.issn0960-0760
dc.identifier.urihttp://repositorio.umaza.edu.ar//handle/00261/1701
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0960076019301013?via%3Dihub
dc.description.abstractARTÍCULO PUBLICADO EN REVISTA EXTERNA. Ovarian carcinoma is one of the most common cause of death by gynecologic cancer. Several epidemiological and in vitro studies have shown controversial data about progesterone effects in ovarian cancer. Progesterone can be converted in its active metabolite, allopregnanolone, its effects in ovarian cancer are still unknown. Previously, we demonstrated that allopregnanolone modifies ovarian morphophysiology, being able to alter critical process of tumor development such as proliferation, apoptosis and angiogenesis. Taking into account these antecedents, we investigated the effect of progesterone and allopregnanolone on proliferation, apoptosis, clonogenic capacity and migration on two epithelial human ovarian cancer cell lines, IGROV-1 and SKOV-3. To this end, IGROV-1 and SKOV-3 cells were exposed to a range of progesterone and allopregnanolone concentrations (10-11 to 10-5 M) for 72 h. Proliferation was analyzed by MTT and Ki67 expression. Apoptosis was measured by immunocytochemistry of cleaved caspase 3. Clonogenic capacity was evaluated by counting colonies. Migration was analyzed by wound assay. We found that allopregnanolone increased proliferation and Ki67 expression respect to control on IGROV-1 cells, while expression of cleaved caspase 3 did not change in any cell line studied. IGROV-1 clonogenic capacity was also increased by allopregnanolone treatment. Both steroids, progesterone and allopregnanolone, increased IGROV-1 migration in a concentration dependent manner. None of the steroids tested modified SKOV-3 biological behavior analized. This is the first evidence that allopregnanolone, a progesterone metabolite, affects critical events in tumor development of human epithelial ovarian cancer. These results could have an impact in the future in clinic diagnosis, prognosis and treatment of ovarian cancer patients. The regulation of progesterone and allopregnanolone steroideogenesis and their molecular mechanisms might be considered as potential therapeutic tool in ovarian cancer. Sitio de la revista: https://www.sciencedirect.com/science/article/pii/S0960076019301013?via%3Dihuben_US
dc.language.isoengen_US
dc.publisherComité editorial Journal of Steroid Biochemistry and Molecular Biologyen_US
dc.source196;
dc.subjectProliferationen_US
dc.subjectClonogenic capacityen_US
dc.subjectMigrationen_US
dc.subjectTumorigenicen_US
dc.subject5α-pregnanesen_US
dc.titleEffect of progesterone and first evidence about allopregnanolone action on the progression of epithelial human ovarian cancer cell linesen_US
dc.typeArtículo Científicoen_US
umaza.description.filiationFil: Pelegina, Laura Tatiana. Laboratorio de Fisio-patología ovárica, Instituto de Medicina y Biología Experimental (IMBECU)- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina. Facultad de Ciencias Médicas y Facultad de Ingeniería, Universidad de Mendoza, Mendoza, Argentina. Universidad Juan Agustín Maza, Mendoza, Argentina.en_US
umaza.description.filiationFil: Sanhueza, María de los Ángeles. Laboratorio de Fisiopatología Ovárica, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU - CONICET), Mendoza, Argentina.en_US
umaza.description.filiationFil: Cáceres, Antonella Rosario Ramona. Laboratorio de Fisio-patología ovárica, Instituto de Medicina y Biología Experimental (IMBECU)- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina. Facultad de Ciencias Médicas y Facultad de Ingeniería, Universidad de Mendoza, Mendoza, Argentina. Universidad Juan Agustín Maza, Mendoza, Argentina.en_US
umaza.description.filiationFil: Cuello Carrión, Dario. Laboratorio de Fisio-patología ovárica, Instituto de Medicina y Biología Experimental (IMBECU)- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza, Argentina.en_US
umaza.description.filiationFil: Rodriguez, Cristina Elisa. Universidad Juan Agustín Maza, Mendoza, Argentina.en_US
umaza.description.filiationFil: Laconi, Myriam Raquel. Laboratorio de Fisiopatología Ovárica, Instituto de Medicina y Biología Experimental de Cuyo (IMBECU - CONICET), Mendoza, Argentina. Facultad de Ciencias Veterinarias y Ambientales, Universidad Juan Agustín Maza, Mendoza, Argentina. Facultad de Ciencias Médicas, Universidad de Mendoza, Mendoza, Argentina.en_US
umaza.statusSNRDPublicadaen_US


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  • Ciencias de la Salud Humana [40]
    Artículos científicos publicados por equipos de investigación UMaza con resultados de proyectos realizados en las áreas disciplinares: ciencias de la salud humana

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